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Animal management practices (i.e., fencing, stocking rate) have been shown to be important factors in the spread of CWD. Stone et al. (2004) found that deer management practices (i.e., fencing, stocking rate) can influence the natural spread of CWD. There was an average of two to three cervids per fence line (some as many as six) within the one-year study period in Colorado. Sites were identified as either having a high or low stocking density. Stocking density appeared to influence the amount of interaction observed between captive and free-ranging cervids within these fenced sites. Cervids with the highest stocking density had the most interaction with wild cervids. Cervids with a stocking density of one to two animals per hectare did not exhibit any fence line interactions. Sites with a lower stocking density had a higher number of cervids per fence line, which again seemed to increase the chances of interactions. Cervids with a stocking density of two to three animals per hectare had the highest level of interaction with wild cervids. The differences in the number of wild cervids at these sites compared to the other sites was most likely due to territorial behavior and the density of cervids. The free-ranging elk herds were much larger than the white-tailed deer herds. Consequently, the elk would most likely have higher densities than the white-tailed deer herds. A possible explanation for the differences in the number of wild cervids at the different sites is that the elk might have higher territorial behavior and so would be less likely to pass the fence line. It is also possible that the stocking density indirectly influences interactions by influencing the availability of prime habitat. The feeding practices at the different sites also seemed to have an effect on interaction. These sites varied in the types of feed provided to the animals in the study. There were two sites that were exclusively fed dry hay (one in Colorado and one in Michigan) and two sites that varied between dry hay and haylage. The sites that provided only hay had the least interaction between captive and free-ranging cervids. Sites that provided hay and haylage had more interaction. Sites which provided only haylage had the lowest stocking density, so the differences in the number of wild cervids could be due to the lack of food source, and more animals and more movement to find food. The sites that provided only haylage appeared to have the most interactions and the most movement.
Thus, the LSM images have revealed the crack behaviors of the Ti film on the PDMS substrates at the micro- and nanoscales. The LSM images are found to be consistent with the optical microscopy images, as shown in Figure 2g,h. From the results obtained so far, it is found that the crack formation mainly occurs during the deformation of the PDMS substrate, and that the crack behaviors are strain dependent. The crack density is larger in the undeformed areas and becomes smaller as the strain becomes larger. 827ec27edc